Imaginary Life-Themes

Malouf's novel is one of deep philosophical themes. The first major theme in an imaginary life is humans' relationship with the natural world. This theme is a central aspect of the novel as it shows the reader how Ovid and the Child's relationship with nature changes their outlooks on life. The closer Ovid becomes with nature the closer he is to achieving inner peace. The same inner peace achieved at the end of the novel when Ovid surrenders himself and becomes one with the world. This is seen through the fact that Ovid becomes immeasurably happy at being the past, present and the future â€Å"It is summer. It is spring. I am immeasurably, unbearably happy. I am three years old. I am sixty. I am six.† This theme is also seen in how inseparable the child is from the natural world and in fact also â€Å"is the natural world†. This is shown in the section of the novel when Ovid is teaching the child how to speak his language. During this time the child also attempts to teach Ovid his language. Ovid finds this as difficult as the child finds learning Ovid's language. Ovid believes that by him â€Å"knowing that it is the sky, that the stars have names and a history† prevents him â€Å"being the sky†. This is due to the fact that Ovid's relationship with the natural world is not yet strong like the child's is. We are shown how crucial the child's relationship with the natural world is through the fact that the child becomes vulnerable and fragile when taken into the settlement in Tomis â€Å"Whatever his secret was I have taken it from him. He is as vulnerable now as anyone of us†¦. He shows himself human at last†. Despite having no dialogue throughout the novel, the function of language is still a central theme of the novel. The relationship between language and perception is highlighted through Ovid's personal descriptions as he believes one cannot view concepts in the same way with different language knowledge. He uses the example of colours to show this on page 25: â€Å"Scarlet. Magic word on the tongue to flash again on the eye. Scarlet†. This describes Ovid's amazement at the feeling evoked from the words. This is also portrayed through Ovid's different perspectives that he describes between the connotations allocated with the abilities of the different languages that he learns. His silent language with the child shows how language can enable one to relate more closely to others and learn from them. Another theme in this novel that helps to describe who the child is is the ‘destiny of the individual.' Throughout the novel Malouf emphasizes that one always has an idea of their destiny. This is illustrated by Ovid's realization that his destiny is lost with the child. Several times Ovid's dreams do in fact unravel themselves in the real world. An example is how he dreams of catching the boy and then later persuades Ryzak the chief to capture him. Another instance where this theory takes effect is in Ovid's dream of going travelling past all â€Å"boundaries† of this world. He later achieves this feet when he is taken across the river Ister by the child. On page 151 Ovid describes the river Ister as his destiny awaiting: â€Å"It remained, shifting its tides, freezing each season, cracking up, flowing again, whispering to me: I am the border beyond which you must go if you are to find your true life, your true death at last.†

Biopure Solution

Biopure Corporation has two new products that are Oxyglobin and Hemopure. Oxyglobin is the first new blood substitute for the veterinary market and is ready for consumer use. Hemopure is another new blood substitute for the human market and it will take two years to launch the product from now on. Ted Jacobs, vice president for Human Clinical Trials, is concerned about creating an unrealistic price expectation for Hemopure by marketing Oxyglobin before Hemopure. On the other hand, Andy Wright, vice president for Veterinary Products, believes that selling Oxyglobin has benefits for the company in terms of generating revenues for the use of launching Hemopure and learning how to market and make mistakes prior to the launch of Hemopure. Carl Rausch, the president and CEO of Biopure Corporation, has to decide if the release of Oxyglobin would be beneficial for the company without jeopardizing the potential of Hemopure. The Solution: I think that launching Oxyglobin has many benefits for the company. For this reason, I agree with Andy Wright’s decision to begin by selling Oxyglobin. Ted Jacobs indicates that the veterinary market is small and price sensitive. He also believes that if the company prices Oxyglobin around $150, it will be very difficult to price Hemopure at $800 because of the huge difference in price for the same product. I disagree with Ted Jacobs because although the production processes and physical characteristics of these two products are identical, Oxyglobin is targeted for the animal market whereas the target customer for Hemopure is the human market. Just because products are identical does not mean that the company prices separately; it is all about the supply and demand. The determining factor of price is the market itself, so Ted Jacobs thinks that Biopure can achieve the price points of $600 to $800. He can only estimate these price points based on the market price. Additionally, even though the veterinary market is small and price sensitive, being the first in any market will make a huge difference and impact the company’s reputation in a positive way if the product satisfies the needs and wants of the consumers. 84% of veterinary doctors complained about the lack of alternatives to the blood transfusion in the marketplace. I believe that this is a big opportunity for Biopure to enter the veterinary market because Oxyglobin has already passed an FDA – approval process specific to the veterinary market. If the competitors want to get a share for animal blood substitutes, they have to wait at least for two years. Waiting for Hemopure to launch both products at the same time will also put the company in a risk because of the possibility of competitors’ entrance to the veterinary market. Once Oxyglobin achieves its purpose for animals and satisfies the veterinary doctors and pet owners, the success of this product will help Biopure to easily launch Hemopure and attract the human market. The Implementation: Andy Wright and his team can have a successful launch for Oxyglobin by implementing the followings: †¢ The Pricing Strategy: Even though the surveys show that veterinarians recommend less-expensive treatments over more-expensive, 90% of pet owners are willing to be informed about all the available alternatives to treat their pets. For this reason, giving the many advantages of Oxyglobin relative to donated animal blood and being the only animal blood substitute in the market, Oxyglobin should carry a premium price of up to $200 per unit. The Distribution Strategy: Instead of making a contract with one of the distributors, Biopure should consider of distributing Oxyglobin by using its salesforces. By doing so, the company does not have to pay 20% of the selling price on a more-established product and 30% of the selling price on a new product. Since the estimated cost of distributing Oxyglobin is $10 to $15 per unit, Biopure would save money compared to the cos t of contracting with one of the distributers which would be $60 per unit.

Application of 2D gas chromatography for environmental analysis

All these benefits make GC?GC in the toxic compounds involved in the determination of trace level environmental analysis of a very good tool in complex matrices. This paper summarizes some of the environmental analysis and review and monitoring of the GC?GC applications Introduction Many years of humans society development led to many of the world distribution of chemicals in the atmosphere, the Earth’s surface and land border. Many of these compounds are harmful to the world’s ecosystems and the people. Analysis of these compounds in the environment is important. When analytes have high vapor pressure, gas chromatography is the selection method. The main problem in the environmental analysis is to analyze the existence of material is usually very complex matrix trace. Result, a huge research work into the analysis of major environmental pollutants [1]. Methods used in environmental analysis is usually the same as in all aspects of practice. It includes sampling, sample preparation, separation and detection. All of these steps may benefit from change, it is usually the biggest limitations imposed by the separation step. In gas chromatography (GC) cases, the majority of environmental samples containing analyte and matrix components of many closely eluting peaks in a chromatographic dimension (1D) the maximum total capacity is greatly exceeded, and many coelutions and unresolved in the separation region was observed. This led to the analyte of interest and quantify the poor separation [1]. Poor resolution in the chromatographic analysis of sample preparation and detection of high demand for equipment placement. Expensive and labor-intensive sample preparation, and solvent waste may cause a lot of harmful to the environment. By microextraction method development, such as liquid-liquid microextraction (LLME) and solid phase microextraction (SPME) and non-dissolved sample introduction system (ie, direct thermal desorption), has the potential to greatly simplify the sample preparation process, without sacrificing sensitivity and selectivity [2-5]. On investigation, lack of resolution often means using mass spectrometry (MS), including high-resolution mass spectrometry (HRMS), in some cases, is necessary. Figure 1 illustrates the GC-MS encountered in the common problems [36]. In the analysis of food extracts commonly 1D-GC clinch with insecticides (Fig. 1b), trace interest (in this case chlorfenvinphos) coelute analyte and sample matrix is more abundant components. Results obtained for such compounds, mass spectrometry (Figure 1e) frequently contains compounds derived from fragments of interference, leading to poor matching and library mass spectrometry (Fig. 1d). MS overlap algorithm may greatly improve the quality of the information of coeluting peak, but they are not always successful, when the number of coelutions is high. Figure 1a shows, full 2D-GC (GC?GC) to increase space and improve the chromatographic separation of the resolution, resulting in the separation of analytes of interest (chlorfenvinphos) from coeluting compounds and matrix components. Result, improved the quality of the analyte mass (Fig. 1c), taking into account the proof of a more confident analysis of material (Figure 1d). It is possible that some coelutions exist; these may often solve efficiently overlap with the MS, leading to better results, while reducing the number of components when the coeluting. GC?GC separation with the increased power resulting in a successful demo nstration and quantification of analytes. Fig. 1 GC?GC–TOF MS versus 1D-GC–TOF MS for the analysis of a carrot extract. The highest-capacity problem in terms of conventional gas chromatography through multi-dimensional gas chromatography to cope (MDGC) implementation. In this method, one-dimensional (1D) chromatogram of a complex and unresolved part is subjected to the stationary phase coated with a second column separation of the other selectivity [1]. Although this method increases the 1D chromatographic part of the choice of chromatographic resolutions, this method with automation challenging, and only a few sample components can be adequately addressed. However, the many applications is good for the PCBs, pesticides and toxaphene analysis, among other things, the report with different degrees of success [6-12]. Overall, however, is the exact number of separation will be beneficial, if the entire sample is subjected to a separation in two dimensions. This became possible a comprehensive two-dimensional gas chromatography (GC?GC) in the introduction. Principle for Two-Dimensional Gas Chromatography (GC?GC) 2D-GC is a comprehensive method of fundamental solution to meet the highest capacity. A typical structure of GC?GC set in Figure 2. The basic structure of GC?GC using virtually is the same as the composition of 1D-GC. These include syringes, oven, columns and detectors. In a typical GC?GC system, using non-polar stationary phase coated with a thick coating of a long column was installed as the main column. The exports through a special interface or modem is connected to the entrance of the second dimension column coated with stationary phase of another selectivity. Modulator connected not only to primary and secondary column; its main role is repeated trapping of the effluent fractions from the first dimension and periodic injection of them to the form of narrow pulses separated into further chromatographic analysis. Because the operation of 2D-GC in the fast condition, the detector in the GC?GC selection is limited to those capable of fast data collection rate. For example, GC?GC de tector can include flame ionization detector (FID), electron capture detector (ECD), single atomic emission detector (AED), sulfur compounds optical detector (SCD), nitrogen photodetector compounds (NCD) and time of flight mass spectrometer (TOF MS). Fig. 2 A block diagram of a GC?GC system. Modulator is the important part of the instrument, because it guarantees the separation is comprehensive and multidimensional [13]. In 1991, the first implementation of the GC?GC, the field has witnessed a number of modulator design [14]. Initially, the use of thermal adjustment of the heat modulator was implemented; however, the modular cryogenic liquid (liquid carbon dioxide or nitrogen) is currently the main use. Modulator at low temperatures within the system, each design has its own distinct advantages and limitations, making it suitable for analysis of the specific type. For example, the analysis of water pollutants has been developed an interface [19], when the buildings, and an in-house applications modulator in the quantitative analysis of PAHs and PCBs has been described [15]. Then, the modulator of the different types of analyte in the analysis of organohalogenated been evaluated [16]. The implementation of GC?GC provides the following advantage to surpass the 1D separation method: improvement separation strength; improved sensitivities; and constructs or highly predetermined, stratography spectrum. In the environment analysis, GC?GC has the potential to improve the toxic compound through the separation from the coeluting analysis and the matrix component, increases the detection limit such chemical product and provides the ideal for the surveillance application the two-dimensional stratography spectrum which constructs. Finally, this possibly causes to reduce to the smallest sample preparation procedure, and reduces analysis time. Other applications are also possible. For example, recently, GC?GC the product estimate which divided into for the diesel oil hydrocarbon environment had used, was important affected many ecosystems [17] the oil leak. Application of GC?GC in Environmental Analysis Water and Sediment Analysis The water is the most basic material to the life in planet. In order to estimate that the tap water safety for human consumption, the rapid, precise and the accurate method needs to analysis the water. The sediment is also important for river and the lake; The analysis of water pollutant is time-consuming sample preparation, follows by GC-MS analyzes. In the initial period realized that GC?GC has the great potential improvement to analysis water and sediment. In its earliest applications in this region, GC?GC was explained possibly from the common matrix interference which is separating the BTEX (benzene, toluene, ethyl benzene and xylene) and methyl alcohol tert butyl ether (MTBE), when and SPME [18]. The separation strength of GC?GC is improved; MTBE and the benzene are the foundation line solution in the 2nd chromatograph analysis space. This research showed GC?GC has the great potential for water pollutant analysis by combination this technology with microextraction (head space SPME). Certain Earth’s freshwater body is polluted daily by petroleum and the oil contamination. In the 1970s, it is pays attention the petroleum sample stratography spectrum to exhibit a model, has not solved, foundation line which rises â€Å"hillock† [20]. Chromatogram is the complex part, including compound many different kinds, refers to â€Å"unsolution complex mixture† at present [21]. GC?GC-FID uses in analysis of two different freshwater sediments [21]. Observed the conventional sample preparation procedure, the author has used the superior resolution, and has constructed the chromatogram of sediment for UCM different levele by GC?GC. The chromatogram obtained for two samples provides by clue direction contamination important source researcher. , The research showed GC?GC the potential importantly in the environment law, for an environmental chemistry basic tool, environmental audit. The nonylphenol polyethylene ethoxides’ degenerated product, was possible feminine hormone splitter [22]. Increases the concern, NPs from the urban district [23] the water and the deposition present are found. GC?GC-TOF MS is the NP isomer separation from technical mixture [24] used. 41 components are identified. Figure 3 explanation GC?GC-TOF MS application to NP isomer respective ion trace analysis from identical research. Two NP stave products were explained that m/z 135 (chart 3a) and m/z 149 (chart 3b). Two chromatogram exhibition group type separation, emphasizes by the connection compound peak maximum value in the identical homologous family incline line. It from as a result of various NP isomer structure similarity, the complete separation is the very difficult this chart is obvious. However, other resolution strength by GC?GC provided â€Å"cleanly† the mass spectrum to provide, made the analysis proof to be easier. Fig. 3. Extracted ion GC?GC–TOF MS chromatograms of a technical nonylphenyl (NP) mixture GC?GC for to the environment pollutant’s analysis was recently the application current in oceanic deposit [25]. A qualitative method has developed, fast and is unified according to the tendency by the ultrasonic wave assistance’s extraction to the complex sample’s high resolution analysis provides to GC?GC-TOF UAE which is fast and high efficiency selective sampling pretreatment procedure is utilized solid sample [30]. A high efficiency and has the selective sample preparation method, when the powerful separation method GC?GC combine with UAE can causes 1500 kind of more than several not aromatic hydrocarbon (PAHs) the compound and the certificate resolution, NPs and dialkylated benzene. Once more, GC?GC not only ability from each other isolation analysis, and has proven from the sample matrix priceless. The carcinogen which and the mutagen suspected, PAHs is many industry activity by-product and the universal existence is distributed in the environment. Because it requests the hard sledding and has the selective sample preparation, they in the deposition sample’s trace determination are difficult. The improvement to complex matrix’s PAHs, the Cavagnino trace analysis with GC?GC-FID [large-volume splitless injection (LVSI) technology]. Sample complex which analyzes is many deposition sample representative who obtains from the river and the lake. Separated and investigates seven PAHs which diluted in the synthesis diesel oil to demonstrate the LVSI- GC?GC-FID potential achievement in the low ppb level for to trace amount analysis one powerful and the rapid tool in complex matrix PAHs. While, Ong. and so on has developed a PAHs rapid surveillance method probably in the soil sample, utilizes liquid extraction (PLE) – GC?GC-FID [27]. The current publishing work is merely GC?GC latent serviceable demonstration to deposition sample PAH analysis. In brief, with the resolution which improves, improvement many sensitivities and the stratography spectrum which orders, GC?GC may add on the result which effective and the rapid sample preparation method produces cannot be achieved by the routine analysis procedure. Analysis of PCBs, PCDDs and PCDFs Polychlorinated dibenzodioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and some polychlorinated biphenyl (PCB) congeners is dominated by bioaccumulation and biomagnification in the environment and thus is a dangerous The wildlife and people. Many are suspected carcinogens and induced changes of [28]. Of PCBs, dioxins and furans in the environment assessment of certain requirements of a method to isolate and quantify them in complex samples such as food, soil and water. GC?GC provides one advantageous method in complex matrix’s PCDDs and the PCDFs analysis. In its one of early experiments, a liquid crystal main column and a limitless secondary column (according to steam pressure separation) uses in (according to the planarity separation) separates the tone – and from technical mixture [30] non-straight PCB congeners. The connection GC?GC microelectron captures investigates (MECD) is toxic PCBs, PCDDs and the PCDFs determination is the application in the cod liver sample [29]. The analysis result showed all 12 priority PCB from liver sample congeners, and most toxic Dai Aoxin and fu nan the full separation and the proof nail fast with 90 PCBs and 17 contain poison PCDDs and PCDFs. Moreover, when compares with the standard sample preparation procedure, the liver sample pretreatment does not have the selectivity and reduces to is smallest. It has included the direct injection and fractionation followed cell degree of illness gradually dra ws back, the centrifuge process to enter GC?GC the system. Figure 4 showing from the 2nd stratography spectrum which obtains to the cod liver sample’s analysis. Recently, an item of multilaboratory research has been conducted in food sample, analyzes PCDD/Fs and World Health Organization PCBs through once more GC?GC-MECD and the GC-HRMS comparison and the explanation GC?GC great potential in the rapid surveillance application [31]. With the standard analysis method comparison GC?GC, GC?GC the performance is unified (GC?GC-ID-TOF MS) has to 13C mark isotopic dilution (ID) TOF MS conventional GC-HRMS to appraise [32]. Quantification 17 PCDD/Fs and four PCBs nail fast in the soil and in the deposition sample are two methods are comparable. However, GC?GC implementation request only smallest sample preparation, and causes the signal improvement (factor 5-10), superior resolution, lower instrumentation expense, and improved TOF the MS data [32] the ghost overlaps legitimately. As highest capacity which and resolution result increases, the unknown compound’s proof is possible. Fig. 4 GC?GC–ECD chromatogram of a cod liver sample spiked with 90 PCBs [29]. Pesticide Analysis Forms the challenge to the pesticide analysis to analyze the chemist to prepare about the sample to make the law and the chromatography. Is similar other toxic compound, the pesticide is usually distributed in the trace amount environment. Moreover, they are extreme complex matrix part of for example foods, the soil and the water sample. Needs to be like today presses to the rapid high resolution analysis method. GC?GC the application early showed the method potential regular implementation to the pesticide analysis in person’s organization in the future. Supercritical invariable extraction (SFE) with GC?GC-FID together utilizes the analysis in person’s blood serum [33] the pesticide. To were few from the sharp person’s blood serum extraction’s 15 pesticide’s foundation line segments achieve in four minutes. Later, GC?GC-FID to estimate that the child pesticide exposure has been utilized through the use urine and the blood serum [34] low-power. This special example in were few showed 16 pesticide complete separations in four minutes. Recently, has been demonstrated including PCBs and the organic chlorine pesticide 59 organization pollutant’s proof and the quantification [35]. But GC?GC-ID-TOF MS completed the comparable result author who ran in standard routine analysis (GC-ID-TOFMS) to indicate that analyzed like this, three different injection needs . GC?GC the application early showed the method potential regular implementation to the pesticide analysis in person’s organization in the future. Supercritical invariable extraction (SFE) with GC?GC-FID together utilizes the analysis in person’s blood serum [33]. Pesticide determination in food extract is similarly important. Separated using GC?GC-TOF MS and identifies 58 pesticides to nail fast completely on the vegetable was explained [36]. This completed with has been smallest and the non-selective sample preparation: The celery or the carrot sample and the sodium acetate and the ethyl acetate have chopped, mixed, was mixed, has been separated, and is dried. The extract is injected entered GC?GC [36]. Recently, separated 12 halogenate compound kind of groups five different GC?GC column combination to appraise, including PCBs, PCDDs, PCDFs, multi-chlorobenzene diphenyl ester (PCDEs), multi-chlorobenzene naphthalene (PCNs), multi-chlorobenzene dibenzothiophenes (PCDTs), multi-chlorobenzene terphenyl (PCTs), multi-chlorobenzene alkane (PCAs), toxaphene, multi-bromination biphenyl (PBBs), multi-bromination diphenyl ether (PBDEs) and organic chlorine pesticide (OCPs) [37]. Although this article focal point is the different compound kind of major group separates, was also explained in the family separation. When the separation and proof all 28 OCPs are pure pesticide mixture has only been demonstrated that majority has been separated fully, since, when injects along other 11 compound kind of [37]. Therefore, its as if that the column establishment which disposes appropriately with one, GC?GC may use takes mainly shields step for the environment sample contamination and along pollutant m any other kind of pesticides, with smallest sample preparation. Air Analysis Volatile organic compound (VOCs) in metropolis photochemical smog [38] the generation plays a strong character. The World Health Organization thought that possibly has to the air granular material’s exposition to the human health [39] the ill effect. But, uncertainty existence about from VOCs health effect in metropolis granular material (PM) [40]. Therefore, requests rapid, reliable and information method guarantee in air pollutant successful surveillance, proof and discovery. Many PAHs and PAHs (oxy-PAHs) which oxidizes is the carcinogen which and the mutagen suspected, with, therefore they are in the metropolis aerosol analysis profitable target analysis. GC?GC-FID and GC?GC four-pole MS (QMS) is applied permits from Finland about 1500 peaks goal PAHs [41] investigates in the metropolis air sample and the proof. But woman is unified the method for the compound proof and the quantification, used GC?GC-FID the combination to confirm the good reproducibility. 13 non-goal PAHs has been identified, and ten goal PAHs by quota. Found PAH centralism scope (0.5-5.5 ng/m3) with in Europe [41] other parts of standard methods obtained the result was comparable. The cigarette smoke is estimate extreme complex mixture component [43] which has not recognized including about 4,700 kind of identification’s compound and 100,000. GC?GC-TOFMS utilizes the solution approximately from the cigarette smoke [43] 30,000 peaks. After this, analyzes cigarette smoke condensate simpler sample determination neutrality score [44], basic score [45] and acidic score [46] chemical composition. Conventional GC-MS possible to separate 200 unknown peaks and identifies 115 hydrocarbons from the cigarette condensate limitless neutral scores; To identical sample GC?GC analysis, however, has achieved 4,000 kind of compound separations and 1,800 hydrocarbons [44] proved. In another research, GC?GC-TOF has identified 377 kind of nitrogen-containing compound to the cigarette condensate’s basic score’s MS analysis, in 155 is the pyridine derivative, 104 kinds kui lin or different kui lin derivative and 56 kind of pyrazine derivative [45]. Conclusions GC?GC has achieved the condition rapidly for to the volatile organic compound analysis most powerful tool. It appoints oneself achievement to be suitable completely for in the complex sample surveillance analysis technology. In the environment analyzed area, this includes PCBs by the analysis many example testimony to the common environment pollutant, PCDDs, PCDFs, PAHs and the pesticide in the complex environment matrix. Moreover, GC?GC has the potential to simplify the sample preparation procedure (even completely to eliminate them), when simultaneously causes when the shorter overall analysis time high resolution stratography spectrum. Regarding widely a new analysis method which adopts, not only it is certainly reliable and renewable, but it should also exhibit the significant advantage to surpass the method which accepts. The example reported the showing GC?GC method advantage in this review in the traditional 1DGC separation. In GC?GC historical first years period, the instrumentation development is the main focal point; However, from GC?GC system’s commercialization, the application quantity which reported greatly increases in the environment analysis and other scientific fields. Therefore, we may anticipate that the transition automation GC?GC is unified on-line sample which the correspondence uses to prepare gradually the equipment in the regular environmental monitoring. References 1. Marriott PJ. Haglund P, Ong RCY. Clin Chim Acta. 2003, 328:1–19. 2. Pawliszyn J. Solid phase microextraction, theory and practice. Wiley, New York. 1997. 3. Pawliszyn J. (1999) Applications of solid phase microextraction. Royal Society of Chemistry, Cambridge. 4. Dettmer K, Engewald W. Anal Bioanal Chem. 2002, 373: 490–500. 5. Butrym E. LC-GC. 1999, 17:S19–S24. 6. de Geus H-J, Wester PG, Schelvis A, de Boer J, Brinkman UATh. J Environ Monit. 2000, 2:503–511. 7. Mrowetz SHJ. J Chromatogr A. 1983, 279:173–187. 8. Duinker JC, Schultz DE, Petrick G. Mar Pollut Bull. 1998, 19:19–25. 9. Storr-Hansen E. Int J Environ Anal Chem.1991, 43:253–266. 10. Silvis LD, Kapila S, Yan Q, Elseewi AA. J Chromatogr A.1994, 688:221–230. 11. Schurig V, Reich S. Chirality. 1998, 10:425–429. 12. Liem DAK. Trends Anal Chem. 1999, 18:499–507. 13. Giddings JC. Anal Chem. 1984, 6:1258A–1270A. 14. Liu Z, Phillips JB. J Chromatogr Sci.1991, 29:227–231. 15. Hyotylainen T, Kallio M, Hartonen K, Jussila M, Palonen S, Riekkola M-L. Anal Chem. 2002, 74:4441–4446. 16. Kristenson EM, Korytar P, Danielsson C, Kallio M, Brandt M, Makela J, Vreuls RJJ, Beens J, Brinkman UATh. J Chromatogr A. 2003, 1019:65–77. 17. Arey JS, Nelson RK, Xu L, Reddy CM. Anal Chem. 2005, 77:7172–7182. 18. Gaines RB, Ledford EB, Stuart JD. J Microcol Sep. 1998, 10:597–604. 19. Beens J, Dalluge J, Adahchour M, Vreuls JJR, Brinkman UATh. J Microcol Sep. 2001, 13:134–140. 20. Blumer M, Souza G, Sass J. Mar Biol. 1970, 5:195–202. 21. Frysinger GS, Gaines RB, Xu L, Reddy CM. Environ Sci Technol. 2003, 37:653–1662. 22. Mueller SO. Anal Bioanal Chem. 2004, 378:582–587. 23. Ieda T, Horii Y, Petrick G, Yamashita N, Ochiai N, Kannan K. Environ Sci Technol. 2005, 39:7202–7207. 24. Moeder M, Martin C, Schlosser D, Harynuk J, Gorecki T. J Chromatogr A. 2006, 1107:233–239. 25. Morales-Munoz S, Vreuls RJJ, Luque de Castro MD. J Chromatogr A. 2005, 1086:122–127. 26. Cavagnino D, Magni P, Zilioli G, Trestianu S. J Chromatogr A. 2003, 1019:211–220. 27. Ong R, Lundstedt S, Haglund P, Marriott P. J Chromatogr A. 2003, 1019:221–232. 28. Schwarzenbach RP, Gschwend PM, Imboden DM. Environmental organic chemistry, 2nd edn. Wiley-Interscience, Hoboken, NJ. 2003. 29. Korytar P, Leonards PEG, de Boer J, Brinkman UATh. J Chromatogr A. 2002, 958:203–218. 30. Haglund P, Harju M, Ong R, Marriott P. J Microcol Sep. 2001, 13:306–311. 31. Danielsson C, Wiberg K, Korytar P, Bergek S, Brinkman UATh, Haglund P. J Chromatogr A. 2005, 1086:61–70. 32. Focant J-F, Reiner EJ, MacPherson K, Kolic T, Sjodin A, Patterson DG Jr, Reese SL, Dorman FL, Cochran J. Talanta. 2004, 63:1231–1240. 33. Liu Z, Sirimanne SR, Patterson DG Jr, Needham LL. Anal Chem. 1994, 66:3086–3092. 34. Dimandja J-M, Grainger J, Patterson DG Jr, Turner WE, Needham LL. J Exp Anal Environ Epidem. 2000, 10:761–768. 35. Focant J-F, Sjodin A, Turner WE, Patterson DG Jr. Anal Chem. 2004, 76:6313–6320. 36. Dalluge J, van Rijn M, Beens J, Vreuls RJJ, Brinkman UATh. J Chromatogr A. 2002, 965:207–217. 37. Korytar P, Leonards PEG, de Boer J, Brinkman UATh. J Chromatogr A. 2005, 1086:29–44. 38. Fowler D, Coyle M, Ashmore MR, Bower J, Williams ML, Smith R, Dollard GJ, Lee DS, Jenkin M, Stedman JR, Cox RA, Derwent RG, Harrison RM, Hewitt CN, Maynard RL, Penkett SA, Weston KJ, Woods PJ, Burgess RA, Anderson R. Fourth Report of the Oxidants Review Group 75-104. UK Department of the Environment, Transport and Regions, London. 1997. 39. WHO. Health aspects of air pollution with particulate matter, ozone and nitrogen dioxide. World Health Organization, Bonn. 2003. 40. Welthagen W, Schnelle-Kreis J, Zimmermann R. J Chromatogr A. 2003, 1019:233–249. 41. Kallio M, Hyotilainen T, Lehtonen M, Jussila ML, Hartonen K, Shimmo M, Riekkola M-L. J Chromatogr A. 2003, 1019:251– 260. 42. Schnelle-Kreis J, Welthagen W, Sklorz M, Zimmermann R. J Sep Sci. 2005, 28:1648–1657. 43. Dalluge J, van Stee LLP, Xu X, Williams J, Beens J, Vreuls RJJ, Brinkman UATh. J Chromatogr A.2002, 974:169–184. 44. Lu X, Zhao M, Kong H, Cai J, Wu J, Wu M, Hua R, Liu J, Xu G. J Chromatogr A. 2004, 1043:265–273. 45. Lu X, Zhao M, Cai J, Kong H, Wu J, Wu M, Hua R, Liu J, Xu G. J Sep Sci. 2004, 27:101–109. 46. Lu X, Cai J, Zhao M, Kong H, Wu J, Wu M, Hua R, Liu J, Xu G. Anal Chem. 2004, 74:4441–4451. Application of 2D gas chromatography for environmental analysis All these benefits make GC?GC in the toxic compounds involved in the determination of trace level environmental analysis of a very good tool in complex matrices. This paper summarizes some of the environmental analysis and review and monitoring of the GC?GC applications Introduction Many years of humans society development led to many of the world distribution of chemicals in the atmosphere, the Earth’s surface and land border. Many of these compounds are harmful to the world’s ecosystems and the people. Analysis of these compounds in the environment is important. When analytes have high vapor pressure, gas chromatography is the selection method. The main problem in the environmental analysis is to analyze the existence of material is usually very complex matrix trace. Result, a huge research work into the analysis of major environmental pollutants [1]. Methods used in environmental analysis is usually the same as in all aspects of practice. It includes sampling, sample preparation, separation and detection. All of these steps may benefit from change, it is usually the biggest limitations imposed by the separation step. In gas chromatography (GC) cases, the majority of environmental samples containing analyte and matrix components of many closely eluting peaks in a chromatographic dimension (1D) the maximum total capacity is greatly exceeded, and many coelutions and unresolved in the separation region was observed. This led to the analyte of interest and quantify the poor separation [1]. Poor resolution in the chromatographic analysis of sample preparation and detection of high demand for equipment placement. Expensive and labor-intensive sample preparation, and solvent waste may cause a lot of harmful to the environment. By microextraction method development, such as liquid-liquid microextraction (LLME) and solid phase microextraction (SPME) and non-dissolved sample introduction system (ie, direct thermal desorption), has the potential to greatly simplify the sample preparation process, without sacrificing sensitivity and selectivity [2-5]. On investigation, lack of resolution often means using mass spectrometry (MS), including high-resolution mass spectrometry (HRMS), in some cases, is necessary. Figure 1 illustrates the GC-MS encountered in the common problems [36]. In the analysis of food extracts commonly 1D-GC clinch with insecticides (Fig. 1b), trace interest (in this case chlorfenvinphos) coelute analyte and sample matrix is more abundant components. Results obtained for such compounds, mass spectrometry (Figure 1e) frequently contains compounds derived from fragments of interference, leading to poor matching and library mass spectrometry (Fig. 1d). MS overlap algorithm may greatly improve the quality of the information of coeluting peak, but they are not always successful, when the number of coelutions is high. Figure 1a shows, full 2D-GC (GC?GC) to increase space and improve the chromatographic separation of the resolution, resulting in the separation of analytes of interest (chlorfenvinphos) from coeluting compounds and matrix components. Result, improved the quality of the analyte mass (Fig. 1c), taking into account the proof of a more confident analysis of material (Figure 1d). It is possible that some coelutions exist; these may often solve efficiently overlap with the MS, leading to better results, while reducing the number of components when the coeluting. GC?GC separation with the increased power resulting in a successful demo nstration and quantification of analytes. Fig. 1 GC?GC–TOF MS versus 1D-GC–TOF MS for the analysis of a carrot extract. The highest-capacity problem in terms of conventional gas chromatography through multi-dimensional gas chromatography to cope (MDGC) implementation. In this method, one-dimensional (1D) chromatogram of a complex and unresolved part is subjected to the stationary phase coated with a second column separation of the other selectivity [1]. Although this method increases the 1D chromatographic part of the choice of chromatographic resolutions, this method with automation challenging, and only a few sample components can be adequately addressed. However, the many applications is good for the PCBs, pesticides and toxaphene analysis, among other things, the report with different degrees of success [6-12]. Overall, however, is the exact number of separation will be beneficial, if the entire sample is subjected to a separation in two dimensions. This became possible a comprehensive two-dimensional gas chromatography (GC?GC) in the introduction. Principle for Two-Dimensional Gas Chromatography (GC?GC) 2D-GC is a comprehensive method of fundamental solution to meet the highest capacity. A typical structure of GC?GC set in Figure 2. The basic structure of GC?GC using virtually is the same as the composition of 1D-GC. These include syringes, oven, columns and detectors. In a typical GC?GC system, using non-polar stationary phase coated with a thick coating of a long column was installed as the main column. The exports through a special interface or modem is connected to the entrance of the second dimension column coated with stationary phase of another selectivity. Modulator connected not only to primary and secondary column; its main role is repeated trapping of the effluent fractions from the first dimension and periodic injection of them to the form of narrow pulses separated into further chromatographic analysis. Because the operation of 2D-GC in the fast condition, the detector in the GC?GC selection is limited to those capable of fast data collection rate. For example, GC?GC de tector can include flame ionization detector (FID), electron capture detector (ECD), single atomic emission detector (AED), sulfur compounds optical detector (SCD), nitrogen photodetector compounds (NCD) and time of flight mass spectrometer (TOF MS). Fig. 2 A block diagram of a GC?GC system. Modulator is the important part of the instrument, because it guarantees the separation is comprehensive and multidimensional [13]. In 1991, the first implementation of the GC?GC, the field has witnessed a number of modulator design [14]. Initially, the use of thermal adjustment of the heat modulator was implemented; however, the modular cryogenic liquid (liquid carbon dioxide or nitrogen) is currently the main use. Modulator at low temperatures within the system, each design has its own distinct advantages and limitations, making it suitable for analysis of the specific type. For example, the analysis of water pollutants has been developed an interface [19], when the buildings, and an in-house applications modulator in the quantitative analysis of PAHs and PCBs has been described [15]. Then, the modulator of the different types of analyte in the analysis of organohalogenated been evaluated [16]. The implementation of GC?GC provides the following advantage to surpass the 1D separation method: improvement separation strength; improved sensitivities; and constructs or highly predetermined, stratography spectrum. In the environment analysis, GC?GC has the potential to improve the toxic compound through the separation from the coeluting analysis and the matrix component, increases the detection limit such chemical product and provides the ideal for the surveillance application the two-dimensional stratography spectrum which constructs. Finally, this possibly causes to reduce to the smallest sample preparation procedure, and reduces analysis time. Other applications are also possible. For example, recently, GC?GC the product estimate which divided into for the diesel oil hydrocarbon environment had used, was important affected many ecosystems [17] the oil leak. Application of GC?GC in Environmental Analysis Water and Sediment Analysis The water is the most basic material to the life in planet. In order to estimate that the tap water safety for human consumption, the rapid, precise and the accurate method needs to analysis the water. The sediment is also important for river and the lake; The analysis of water pollutant is time-consuming sample preparation, follows by GC-MS analyzes. In the initial period realized that GC?GC has the great potential improvement to analysis water and sediment. In its earliest applications in this region, GC?GC was explained possibly from the common matrix interference which is separating the BTEX (benzene, toluene, ethyl benzene and xylene) and methyl alcohol tert butyl ether (MTBE), when and SPME [18]. The separation strength of GC?GC is improved; MTBE and the benzene are the foundation line solution in the 2nd chromatograph analysis space. This research showed GC?GC has the great potential for water pollutant analysis by combination this technology with microextraction (head space SPME). Certain Earth’s freshwater body is polluted daily by petroleum and the oil contamination. In the 1970s, it is pays attention the petroleum sample stratography spectrum to exhibit a model, has not solved, foundation line which rises â€Å"hillock† [20]. Chromatogram is the complex part, including compound many different kinds, refers to â€Å"unsolution complex mixture† at present [21]. GC?GC-FID uses in analysis of two different freshwater sediments [21]. Observed the conventional sample preparation procedure, the author has used the superior resolution, and has constructed the chromatogram of sediment for UCM different levele by GC?GC. The chromatogram obtained for two samples provides by clue direction contamination important source researcher. , The research showed GC?GC the potential importantly in the environment law, for an environmental chemistry basic tool, environmental audit. The nonylphenol polyethylene ethoxides’ degenerated product, was possible feminine hormone splitter [22]. Increases the concern, NPs from the urban district [23] the water and the deposition present are found. GC?GC-TOF MS is the NP isomer separation from technical mixture [24] used. 41 components are identified. Figure 3 explanation GC?GC-TOF MS application to NP isomer respective ion trace analysis from identical research. Two NP stave products were explained that m/z 135 (chart 3a) and m/z 149 (chart 3b). Two chromatogram exhibition group type separation, emphasizes by the connection compound peak maximum value in the identical homologous family incline line. It from as a result of various NP isomer structure similarity, the complete separation is the very difficult this chart is obvious. However, other resolution strength by GC?GC provided â€Å"cleanly† the mass spectrum to provide, made the analysis proof to be easier. Fig. 3. Extracted ion GC?GC–TOF MS chromatograms of a technical nonylphenyl (NP) mixture GC?GC for to the environment pollutant’s analysis was recently the application current in oceanic deposit [25]. A qualitative method has developed, fast and is unified according to the tendency by the ultrasonic wave assistance’s extraction to the complex sample’s high resolution analysis provides to GC?GC-TOF UAE which is fast and high efficiency selective sampling pretreatment procedure is utilized solid sample [30]. A high efficiency and has the selective sample preparation method, when the powerful separation method GC?GC combine with UAE can causes 1500 kind of more than several not aromatic hydrocarbon (PAHs) the compound and the certificate resolution, NPs and dialkylated benzene. Once more, GC?GC not only ability from each other isolation analysis, and has proven from the sample matrix priceless. The carcinogen which and the mutagen suspected, PAHs is many industry activity by-product and the universal existence is distributed in the environment. Because it requests the hard sledding and has the selective sample preparation, they in the deposition sample’s trace determination are difficult. The improvement to complex matrix’s PAHs, the Cavagnino trace analysis with GC?GC-FID [large-volume splitless injection (LVSI) technology]. Sample complex which analyzes is many deposition sample representative who obtains from the river and the lake. Separated and investigates seven PAHs which diluted in the synthesis diesel oil to demonstrate the LVSI- GC?GC-FID potential achievement in the low ppb level for to trace amount analysis one powerful and the rapid tool in complex matrix PAHs. While, Ong. and so on has developed a PAHs rapid surveillance method probably in the soil sample, utilizes liquid extraction (PLE) – GC?GC-FID [27]. The current publishing work is merely GC?GC latent serviceable demonstration to deposition sample PAH analysis. In brief, with the resolution which improves, improvement many sensitivities and the stratography spectrum which orders, GC?GC may add on the result which effective and the rapid sample preparation method produces cannot be achieved by the routine analysis procedure. Analysis of PCBs, PCDDs and PCDFs Polychlorinated dibenzodioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and some polychlorinated biphenyl (PCB) congeners is dominated by bioaccumulation and biomagnification in the environment and thus is a dangerous The wildlife and people. Many are suspected carcinogens and induced changes of [28]. Of PCBs, dioxins and furans in the environment assessment of certain requirements of a method to isolate and quantify them in complex samples such as food, soil and water. GC?GC provides one advantageous method in complex matrix’s PCDDs and the PCDFs analysis. In its one of early experiments, a liquid crystal main column and a limitless secondary column (according to steam pressure separation) uses in (according to the planarity separation) separates the tone – and from technical mixture [30] non-straight PCB congeners. The connection GC?GC microelectron captures investigates (MECD) is toxic PCBs, PCDDs and the PCDFs determination is the application in the cod liver sample [29]. The analysis result showed all 12 priority PCB from liver sample congeners, and most toxic Dai Aoxin and fu nan the full separation and the proof nail fast with 90 PCBs and 17 contain poison PCDDs and PCDFs. Moreover, when compares with the standard sample preparation procedure, the liver sample pretreatment does not have the selectivity and reduces to is smallest. It has included the direct injection and fractionation followed cell degree of illness gradually dra ws back, the centrifuge process to enter GC?GC the system. Figure 4 showing from the 2nd stratography spectrum which obtains to the cod liver sample’s analysis. Recently, an item of multilaboratory research has been conducted in food sample, analyzes PCDD/Fs and World Health Organization PCBs through once more GC?GC-MECD and the GC-HRMS comparison and the explanation GC?GC great potential in the rapid surveillance application [31]. With the standard analysis method comparison GC?GC, GC?GC the performance is unified (GC?GC-ID-TOF MS) has to 13C mark isotopic dilution (ID) TOF MS conventional GC-HRMS to appraise [32]. Quantification 17 PCDD/Fs and four PCBs nail fast in the soil and in the deposition sample are two methods are comparable. However, GC?GC implementation request only smallest sample preparation, and causes the signal improvement (factor 5-10), superior resolution, lower instrumentation expense, and improved TOF the MS data [32] the ghost overlaps legitimately. As highest capacity which and resolution result increases, the unknown compound’s proof is possible. Fig. 4 GC?GC–ECD chromatogram of a cod liver sample spiked with 90 PCBs [29]. Pesticide Analysis Forms the challenge to the pesticide analysis to analyze the chemist to prepare about the sample to make the law and the chromatography. Is similar other toxic compound, the pesticide is usually distributed in the trace amount environment. Moreover, they are extreme complex matrix part of for example foods, the soil and the water sample. Needs to be like today presses to the rapid high resolution analysis method. GC?GC the application early showed the method potential regular implementation to the pesticide analysis in person’s organization in the future. Supercritical invariable extraction (SFE) with GC?GC-FID together utilizes the analysis in person’s blood serum [33] the pesticide. To were few from the sharp person’s blood serum extraction’s 15 pesticide’s foundation line segments achieve in four minutes. Later, GC?GC-FID to estimate that the child pesticide exposure has been utilized through the use urine and the blood serum [34] low-power. This special example in were few showed 16 pesticide complete separations in four minutes. Recently, has been demonstrated including PCBs and the organic chlorine pesticide 59 organization pollutant’s proof and the quantification [35]. But GC?GC-ID-TOF MS completed the comparable result author who ran in standard routine analysis (GC-ID-TOFMS) to indicate that analyzed like this, three different injection needs . GC?GC the application early showed the method potential regular implementation to the pesticide analysis in person’s organization in the future. Supercritical invariable extraction (SFE) with GC?GC-FID together utilizes the analysis in person’s blood serum [33]. Pesticide determination in food extract is similarly important. Separated using GC?GC-TOF MS and identifies 58 pesticides to nail fast completely on the vegetable was explained [36]. This completed with has been smallest and the non-selective sample preparation: The celery or the carrot sample and the sodium acetate and the ethyl acetate have chopped, mixed, was mixed, has been separated, and is dried. The extract is injected entered GC?GC [36]. Recently, separated 12 halogenate compound kind of groups five different GC?GC column combination to appraise, including PCBs, PCDDs, PCDFs, multi-chlorobenzene diphenyl ester (PCDEs), multi-chlorobenzene naphthalene (PCNs), multi-chlorobenzene dibenzothiophenes (PCDTs), multi-chlorobenzene terphenyl (PCTs), multi-chlorobenzene alkane (PCAs), toxaphene, multi-bromination biphenyl (PBBs), multi-bromination diphenyl ether (PBDEs) and organic chlorine pesticide (OCPs) [37]. Although this article focal point is the different compound kind of major group separates, was also explained in the family separation. When the separation and proof all 28 OCPs are pure pesticide mixture has only been demonstrated that majority has been separated fully, since, when injects along other 11 compound kind of [37]. Therefore, its as if that the column establishment which disposes appropriately with one, GC?GC may use takes mainly shields step for the environment sample contamination and along pollutant m any other kind of pesticides, with smallest sample preparation. Air Analysis Volatile organic compound (VOCs) in metropolis photochemical smog [38] the generation plays a strong character. The World Health Organization thought that possibly has to the air granular material’s exposition to the human health [39] the ill effect. But, uncertainty existence about from VOCs health effect in metropolis granular material (PM) [40]. Therefore, requests rapid, reliable and information method guarantee in air pollutant successful surveillance, proof and discovery. Many PAHs and PAHs (oxy-PAHs) which oxidizes is the carcinogen which and the mutagen suspected, with, therefore they are in the metropolis aerosol analysis profitable target analysis. GC?GC-FID and GC?GC four-pole MS (QMS) is applied permits from Finland about 1500 peaks goal PAHs [41] investigates in the metropolis air sample and the proof. But woman is unified the method for the compound proof and the quantification, used GC?GC-FID the combination to confirm the good reproducibility. 13 non-goal PAHs has been identified, and ten goal PAHs by quota. Found PAH centralism scope (0.5-5.5 ng/m3) with in Europe [41] other parts of standard methods obtained the result was comparable. The cigarette smoke is estimate extreme complex mixture component [43] which has not recognized including about 4,700 kind of identification’s compound and 100,000. GC?GC-TOFMS utilizes the solution approximately from the cigarette smoke [43] 30,000 peaks. After this, analyzes cigarette smoke condensate simpler sample determination neutrality score [44], basic score [45] and acidic score [46] chemical composition. Conventional GC-MS possible to separate 200 unknown peaks and identifies 115 hydrocarbons from the cigarette condensate limitless neutral scores; To identical sample GC?GC analysis, however, has achieved 4,000 kind of compound separations and 1,800 hydrocarbons [44] proved. In another research, GC?GC-TOF has identified 377 kind of nitrogen-containing compound to the cigarette condensate’s basic score’s MS analysis, in 155 is the pyridine derivative, 104 kinds kui lin or different kui lin derivative and 56 kind of pyrazine derivative [45]. Conclusions GC?GC has achieved the condition rapidly for to the volatile organic compound analysis most powerful tool. It appoints oneself achievement to be suitable completely for in the complex sample surveillance analysis technology. In the environment analyzed area, this includes PCBs by the analysis many example testimony to the common environment pollutant, PCDDs, PCDFs, PAHs and the pesticide in the complex environment matrix. Moreover, GC?GC has the potential to simplify the sample preparation procedure (even completely to eliminate them), when simultaneously causes when the shorter overall analysis time high resolution stratography spectrum. Regarding widely a new analysis method which adopts, not only it is certainly reliable and renewable, but it should also exhibit the significant advantage to surpass the method which accepts. The example reported the showing GC?GC method advantage in this review in the traditional 1DGC separation. In GC?GC historical first years period, the instrumentation development is the main focal point; However, from GC?GC system’s commercialization, the application quantity which reported greatly increases in the environment analysis and other scientific fields. Therefore, we may anticipate that the transition automation GC?GC is unified on-line sample which the correspondence uses to prepare gradually the equipment in the regular environmental monitoring. References 1. Marriott PJ. Haglund P, Ong RCY. Clin Chim Acta. 2003, 328:1–19. 2. Pawliszyn J. Solid phase microextraction, theory and practice. Wiley, New York. 1997. 3. Pawliszyn J. (1999) Applications of solid phase microextraction. Royal Society of Chemistry, Cambridge. 4. Dettmer K, Engewald W. Anal Bioanal Chem. 2002, 373: 490–500. 5. Butrym E. LC-GC. 1999, 17:S19–S24. 6. de Geus H-J, Wester PG, Schelvis A, de Boer J, Brinkman UATh. J Environ Monit. 2000, 2:503–511. 7. Mrowetz SHJ. J Chromatogr A. 1983, 279:173–187. 8. Duinker JC, Schultz DE, Petrick G. Mar Pollut Bull. 1998, 19:19–25. 9. Storr-Hansen E. Int J Environ Anal Chem.1991, 43:253–266. 10. Silvis LD, Kapila S, Yan Q, Elseewi AA. J Chromatogr A.1994, 688:221–230. 11. Schurig V, Reich S. Chirality. 1998, 10:425–429. 12. Liem DAK. Trends Anal Chem. 1999, 18:499–507. 13. Giddings JC. Anal Chem. 1984, 6:1258A–1270A. 14. Liu Z, Phillips JB. J Chromatogr Sci.1991, 29:227–231. 15. Hyotylainen T, Kallio M, Hartonen K, Jussila M, Palonen S, Riekkola M-L. Anal Chem. 2002, 74:4441–4446. 16. Kristenson EM, Korytar P, Danielsson C, Kallio M, Brandt M, Makela J, Vreuls RJJ, Beens J, Brinkman UATh. J Chromatogr A. 2003, 1019:65–77. 17. Arey JS, Nelson RK, Xu L, Reddy CM. Anal Chem. 2005, 77:7172–7182. 18. Gaines RB, Ledford EB, Stuart JD. J Microcol Sep. 1998, 10:597–604. 19. Beens J, Dalluge J, Adahchour M, Vreuls JJR, Brinkman UATh. J Microcol Sep. 2001, 13:134–140. 20. Blumer M, Souza G, Sass J. Mar Biol. 1970, 5:195–202. 21. Frysinger GS, Gaines RB, Xu L, Reddy CM. Environ Sci Technol. 2003, 37:653–1662. 22. Mueller SO. Anal Bioanal Chem. 2004, 378:582–587. 23. Ieda T, Horii Y, Petrick G, Yamashita N, Ochiai N, Kannan K. Environ Sci Technol. 2005, 39:7202–7207. 24. Moeder M, Martin C, Schlosser D, Harynuk J, Gorecki T. J Chromatogr A. 2006, 1107:233–239. 25. Morales-Munoz S, Vreuls RJJ, Luque de Castro MD. J Chromatogr A. 2005, 1086:122–127. 26. Cavagnino D, Magni P, Zilioli G, Trestianu S. J Chromatogr A. 2003, 1019:211–220. 27. Ong R, Lundstedt S, Haglund P, Marriott P. J Chromatogr A. 2003, 1019:221–232. 28. Schwarzenbach RP, Gschwend PM, Imboden DM. Environmental organic chemistry, 2nd edn. Wiley-Interscience, Hoboken, NJ. 2003. 29. Korytar P, Leonards PEG, de Boer J, Brinkman UATh. J Chromatogr A. 2002, 958:203–218. 30. Haglund P, Harju M, Ong R, Marriott P. J Microcol Sep. 2001, 13:306–311. 31. Danielsson C, Wiberg K, Korytar P, Bergek S, Brinkman UATh, Haglund P. J Chromatogr A. 2005, 1086:61–70. 32. Focant J-F, Reiner EJ, MacPherson K, Kolic T, Sjodin A, Patterson DG Jr, Reese SL, Dorman FL, Cochran J. Talanta. 2004, 63:1231–1240. 33. Liu Z, Sirimanne SR, Patterson DG Jr, Needham LL. Anal Chem. 1994, 66:3086–3092. 34. Dimandja J-M, Grainger J, Patterson DG Jr, Turner WE, Needham LL. J Exp Anal Environ Epidem. 2000, 10:761–768. 35. Focant J-F, Sjodin A, Turner WE, Patterson DG Jr. Anal Chem. 2004, 76:6313–6320. 36. Dalluge J, van Rijn M, Beens J, Vreuls RJJ, Brinkman UATh. J Chromatogr A. 2002, 965:207–217. 37. Korytar P, Leonards PEG, de Boer J, Brinkman UATh. J Chromatogr A. 2005, 1086:29–44. 38. Fowler D, Coyle M, Ashmore MR, Bower J, Williams ML, Smith R, Dollard GJ, Lee DS, Jenkin M, Stedman JR, Cox RA, Derwent RG, Harrison RM, Hewitt CN, Maynard RL, Penkett SA, Weston KJ, Woods PJ, Burgess RA, Anderson R. Fourth Report of the Oxidants Review Group 75-104. UK Department of the Environment, Transport and Regions, London. 1997. 39. WHO. Health aspects of air pollution with particulate matter, ozone and nitrogen dioxide. World Health Organization, Bonn. 2003. 40. Welthagen W, Schnelle-Kreis J, Zimmermann R. J Chromatogr A. 2003, 1019:233–249. 41. Kallio M, Hyotilainen T, Lehtonen M, Jussila ML, Hartonen K, Shimmo M, Riekkola M-L. J Chromatogr A. 2003, 1019:251– 260. 42. Schnelle-Kreis J, Welthagen W, Sklorz M, Zimmermann R. J Sep Sci. 2005, 28:1648–1657. 43. Dalluge J, van Stee LLP, Xu X, Williams J, Beens J, Vreuls RJJ, Brinkman UATh. J Chromatogr A.2002, 974:169–184. 44. Lu X, Zhao M, Kong H, Cai J, Wu J, Wu M, Hua R, Liu J, Xu G. J Chromatogr A. 2004, 1043:265–273. 45. Lu X, Zhao M, Cai J, Kong H, Wu J, Wu M, Hua R, Liu J, Xu G. J Sep Sci. 2004, 27:101–109. 46. Lu X, Cai J, Zhao M, Kong H, Wu J, Wu M, Hua R, Liu J, Xu G. Anal Chem. 2004, 74:4441–4451.

Volcano Essay Example | Topics and Well Written Essays - 750 words

Volcano - Essay Example Hot clouds and glowing lava advanced 1 km. down the Genol River. Inhabitants in Muntilan saw the ash fall over surrounding areas. Merapi is considered as the most active of the 130 active volcanoes of Indonesia. Twelve major eruptions have killed thousands. People of Indonesia look at Merapi with awe, wonder and reverence and every year a priest makes the ascent to the top of the mountain to make the prescribed offering. For the last two weeks Merapi volcano has been spewing thick black smoke and the scientists say that a full scale eruption is soon on the cards. The full name of this mountain is Gunung Merapi, meaning Fiery Mountain. It is situated overlooking the ancient royal city of Yogyakarta. Eruptions are a regular feature with Merapi, one of the most active volcanos in the world. Small eruptions take place every 2-3 years, bigger ones at the interval of 10-15 years and the real big ones with tremendous destructive capacity, once in 50-60 years. The eruption of 1006 was so devastating that an entire Hindu Kingdom was destroyed. In 1930, about 1300 people were killed. The 1994 eruption was comparatively less destructive and it claimed 64 lives. Local people consider this volcano as very sacred. They also firmly believe that a supernatural Kingdom exists on the top of Merapi. Merapi is located in the zone called a â€Å"Ring of Fire,† in the Pacific Ocean. When at peace, Merapi is just like a friend to the people and to the tourists who are fond of hiking and climbing. But nothing could be predicted about this wonder of nature. Suddenly you can see the volcanic ash spew and then glowing lava ooze down on all its sides. As it intensifies, the sight of large plume of smoke billowing from it is not only amazing but terrifying! Notwithstanding its fearsome activities, people around the mountain love and respect Merapi and many cultivate crops quite close to the cone section. On November 22, 1994, Merapi shook and was in for a major bout of

The impact of users' interface on users trust in e commerce Essay

The impact of users' interface on users trust in e commerce - Essay Example (p. 920) For online transactions wherein customers buy products and conduct their financial activities, trust is everything. Ecommerce is deeply interwoven with risks for consumers that is why organizations have to work hard in order to earn it. One of the most important features in the design of ecommerce website that achieves for an organization the trust of its visitors is the user interface. (Lumsden, p. 867) This paper will explore and examine this area and, specifically, will outline the relationship between user interface and trust. Consumers’ trust in an e-retailer, wrote Zhou (2006), can be defined as the consumers’ willingness to accept vulnerability in an online transaction based on their positive expectations in regard with and E-retailers future behavior. (p. 335) This is mainly achieved through a positive relationship that are sustained through time. The behavior of E-retailer towards its consumers can both do and undo this. Smith and Salvendy (2007) explained that trust is based upon perception, the process by which individual’s select, filter, organize and interpret information in order to create a meaningful picture of the world. (p. 68) What this highlights is that trust falls under the behavioral and psychological sphere, wherein individuals are affected by and react to symbols and signs that enable them to perceive dependability, credibility and competence. In applying this in ecommerce, one could say that those people, who encounter symbols, read them through their own individual eyes and the symbols acquire meaning in the organization through recurring experiences. (Smith and Salvendy, p. 68) In user interface, integrating this behavioral and psychological consideration would be able to increase value for the website including its trust warranting properties. The trust requirements in ecommerce have produced several guidelines and standards in regard to user interface development and design.

Imagine that you are hanny write a letter to the roadman thank him for Essay

Imagine that you are hanny write a letter to the roadman thank him for his help tell him your real reason for doing this job - Essay Example A few months ago I met a gentleman by the name of Franklin P Scudder, who was in the knowledge of a German plot against England and who solicited my services. Sad to say, but when I returned to my premises after some time, I found Scudder murdered in cold blood. I decided to run away into hiding for many reasons. First I was scared that the people who murdered Scudder will also murder me. Second, I believed that the police will suspect me for the murder of Scudder. Third I wanted to get to the depth of the conspiracy narrated to me by Scudder. When I met you, I was actually being pursued by both the police and the German spies. It was only because of your help that I survived and managed to unravel this conspiracy against Britain, well in time. Thanks a lot for whatever you did for me and the country. Sincere regards, Richard Hannay.

Choose one element that you notice in (Agamemnon, Hamlet, The Love Essay

Choose one element that you notice in (Agamemnon, Hamlet, The Love Song of J. Alfred Prufrock). Explain the similarities and differences as you compare the development in each work of your chosen element - Essay Example To adequately enjoy and gain insights into these works, the reader should endeavor to discern all the notable similarities and differences in these pieces of literature. Hamlet is a story line made famous by William Shakespeare who wrote the plot of the story to be depicted as a play, which makes it a world acclaimed piece of literature and theatrical prose. Hamlet is bent on avenging his father’s death, and this exposes the reader to other aspects of the protagonist’s character that can be construed to be part of the author’s intention and insight to the reader. Hamlet’s vengeance exposes his insecurity leading him to commit incestuous acts with his kin in an effort to secure his dominance and authority. This is an aspect that is present in the ‘The Love Song of J. Alfred Prufock’ in the sense that his insecurities lead him to reveal his inner-self unconsciously. The difference between the two is that in Hamlet, Prince Hamlet quest is fulfilled with the murder of his Uncle the focus of his revenge and hatred. Unlike Hamlet, J. Alfred Prufock’s insecurities and inadequacies haunt him for the rest of th e poem, and the reader is left wondering or yearning to know the outcome or the true end of the story. Agamemnon as a play also presents a dilemma, which is also present in all the literal works under discussion (Aeschylu, Raeburn and Thomas 118). Hamlet is in a dilemma on how he is going to redeem himself in the eyes of his people as their king in the presence of his overbearing uncle. He is also unsure of his mother’s involvement in the murder of his father due to her speedy remarriage to his uncle whom he suspects as his father’s killer (Crowl 169). Agamemnon is in a dilemma as to the future of his position upon his return from the Trojan War, an aspects he battles with till his ultimate death or murder. The

Statement of Purpose Essay Example | Topics and Well Written Essays - 1000 words - 7

Statement of Purpose - Essay Example er, the element finance run in my family as my mother and father handles their own business and my education in finance would aid both of them considerably. It is my mother’s trade in Gold at metatrader software program that invoked me to start a finance related study .On one winter break, I voluntarily started studying the trade market, accustomed myself with the finance news, updated forex calendar, indulged in online trading chart and scrutinized technical analyzing tools. All of these sparked in me a passion to learn more about finance, especially quantitative finance. The uncertainty and fluctuation in the financial field pulled me strongly into it as I found it to be really challenging. This was the moment of truth and I decided to take up a credible course in quantitative financial studies. At the same time, I also realized the importance of finance in this sophisticated world and how much this study can help me in building my future. My excitement in learning finance related course soon turned out into a consolidated passion and solidified my desire to devote my future to the field of finance and investment. The quantitative finance course offered by your esteemed organization impressed me greatly and convinced me to get admission in it in a chase to follow up my dreams and desires. I understood that this quantitative program has the potentiality to manage assets and minimize risk which is the core aim of any business related study. I foresee that finance related course has the power to destroy or flourish the status of a business. This even more strengthened my decision to learn quantitative finance course. Moreover, it is when the financial crisis hit the world in 2009, I completely understood the importance of financial study on an international platform. I would like to confess here that the appropriate course offered by your prestige university has the impeccable ability to construct my future in a positive manner. I can put down in front of you

What is on the Horizon for Apple Research Paper

What is on the Horizon for Apple - Research Paper Example Users were given choice to buy single songs instead of buying a complete audio disc. The iPod was different than an already saturated mp3 player market at the time. It offered superior user interface and hence better user experience. The iPod represents a start for the company to lead the revolution. The innovations did not stop and new products that invented completely new categories followed. Apple plans to grow their product lineup as well as improve existing products. Some of the possible future plans are discussed in detail; Mac Macs have been the main product for Apple since long. The company was mainly founded as a computer company. The lineup includes Mac Pro, iMac, Mac mini, MacBook Pro and MacBook Air. Macs currently run on Intel processors. MacBook Pro and MacBook Air represents major portion of the sales in Macs. Both products are made with unibody enclosures, which add durability and aesthetics. The MacBook Airs are ultra thin laptops running on low power processors and incorporate Solid State Drives (SSD) instead of convention Hard Disc Drives used in MacBook Pros. Apple plans to introduce high resolution Retina Displays in both MacBooks (Chen & Shen, 2011). This will improve real estate available on desktop as well as improve sharpness, in particular for texts. Furthermore, MacBook Pro is rumored to have a design overhaul in 2012 and will adopt design cues from the existing MackBook Air (Kim, 2012). The two lines of models are expected to merge into one product in the future. iPad The iPad represents the leading post-pc device from Apple. Apple invented a completely new category with the introduction of iPad in 2010. The tablet has been an astounding success and is liked for its ease of use and portability. The iPad has capitalized the tablet market till now and Apple has dominated the category. Competitors have introduced Android based tablets of varying size and price to compete against the iPad. It is rumored that Apple is planning to introduc e 7.85 inch iPad variant as compared to 9.7 inch current iPad’s screen size (Faust, 2012). The 7 inch screen size was dismissed by late Apple CEO Steve Jobs to be very small and incapable of expressing the software. Though, with the new iPad carrying Retina Display (resolution 2048 x 1536 pixels), there is a possibility that Apple may introduce 7.85 inch variant carrying a resolution of 1024 x 768 pixels. The major competitor to the iPad would be Windows 8 tablets planned at the end of 2012. The Windows 8 operating system would be a complete operating system working on a tablet device as compared to relatively limited mobile based Apple’s iOS on the iPad. For now, sales of the new iPad are phenomenal and Apple sold record breaking 3 million iPads in first four days of launch (Golson, 2012). iPhone The original iPhone introduced in 2007 revolutionized the mobile phone industry and left competitors struggling to cope up with its advanced features, usability and user expe rience. Over the years the phone market has become saturated with Android based devices and manufacturers offer various type of phones based on the platform. Apple’s iOS on the other hand has been a stable platform and has standard parameters. Android is running on devices with varying hardware parameters and is, therefore, fragmented. This means that applications are not designed to run on every phone with varying hardware specifications. For the next generation iPhones Apple plans to improve its camera with an aim to replace everyday point and shoot cameras. The next iPhone is rumored to have

Consumer Society Gives People Choice Essay Example | Topics and Well Written Essays - 1500 words

Consumer Society Gives People Choice - Essay Example Hinchcliff et al, (2009) see consumption as a lifestyle and something that comes as a result of socialization. It is therefore, for him, a lifestyle involving people using goods and services, as the last bearers. It therefore for him the end line of ‘economic activities’ begins with assessment of the resources available and goes on through to the production stage and distribution.Consumer Society, or rather Consumerism, has been viewed as an economic as well as a social structure tailored and lined on the calculated systematic creation and fostering of a desire to purchase goods and services in greater amounts and sometimes with a tinge of variety. It is a process that sows the seed of belongingness (Hinchcliff et al, 2009, pg 20).The major understanding by social scientists is that consumption presents different values, attitudes and a sense of belonging (Hinchcliff et al, 2009, pg 21). The characteristic of the consumer society can be examined in the context of the com mon expression that support the concept consumer society and the lifestyle that individual and collective consumers exhibit as far postmodern consumerism is concerned. For Social Scientists, a number of factors come into play as far as consumer choices go. These include income, media, goods and their nature, educational status, social expectations, peer influence among others. Some of the variables to these would perhaps be captured in the popular expressions would be like ‘everybody is a walking advertisement’.... It is a process that sows the seed of belongingness (Hinchcliff et al, 2009, pg 20). Features of the Consumer Society The major understanding by social scientists is that consumption presents different values, attitudes and a sense of belonging (Hinchcliff et al, 2009, pg 21). The characteristic of the consumer society can be examined in the context of the common expression that support the concept consumer society and the lifestyle that individual and collective consumers exhibit as far postmodern consumerism is concerned. For Social Scientists, a number of factors come into play as far as consumer choices go. These include income, media, goods and their nature, educational status, social expectations, peer influence among others (Hinchcliff et al, 2009, pg 21). Some of the variables to these would perhaps be captured in the popular expressions would be like ‘everybody is a walking advertisement’. Consumer Sovereignty (Hinchcliff et al (2009) seems to contend that that from the time of the Economists Adam Smith, economics debates have always had the assumption that the centre of a sound economy is based on the final demand of goods/services; therefore, it is believed my man. Economists argue that meeting the demands involves amicably seeking to ensure that their desires are met and that they are centre of production and promotion of conspicuous buying and therefore fitting the label â€Å"these are the seduced ones of the nineteenth century† (Hinchcliff et al, 2009, pg 32). Arguments for the Consumer Sovereignty: What are some of the causes and institutions? The basic assumption here is that the consumer in the modern

Knowing Your Audience and Communication Release Essay Example for Free

Knowing Your Audience and Communication Release Essay On August 5, 2010, The Chilean Copper mine collapse with 33 workers confined in a 2,257 feet hole, which was the size of a mini studio apartment (msnbc.com news services, 2010). Their families, friends, and co-workers were worried frantically about their safety, and not being able to reach the miners left everyone at unease. The Rescuers and the Chilean Copper president’s came together to find a resolution as to the cause of this situation and how to rescue their employees. Families and co-workers of these miners have questions that wanted immediate answers, and it is the companies responsibly to provide answers. The company should have a plan of emergency action for these types of incident and be implemented in training programs when hired or promoted. There should be reputable spokesperson on hand to communicate with families, co-workers, and the media. The first action to take place is to immediately control the situation, and prevent any rumors from uprising through the workplace or the media. Have somebody Reputable from the company to hold a face to face verbal meeting explaining what emergency incident just occurred and what the companies’ plan of action is going to be. It is important to present only facts to this meeting so that there are no assumptions as to what is really taking place. Be honest, open, clear and concise in these meeting so the audience can listen and decode the message the spokesperson is conveying. The spokesperson should be able to answer all questions presented to the room, as long as the spokesperson has evidence to back it up. Otherwise, being honest and saying, â€Å"we are looking into that,† is the best option for this type of situation. If the company has a morning and evening shift, then having the meeting write when both shifts are about to cross would be an effective way to control any rumors, explain the situation, and answer any questions. This will save time so the company can delegate the situation currently on hand. The second action is to have another reputable spokesperson to notify the minors’ families of the incident. The first step to communicating to the miners loved ones is by phone and then a face-to-face verbal communication should take place. It is important to be sympathetic and compassionate when relaying this type of information to the families. Calling each miners emergency contact would be the start but should not provide too much information over the phone, but just ask the family or friend to come down to the job site immediately. Providing information over the phone can create media attention rapidly and cause panic to the person. Hold a meeting as soon as the miner’s family and friends have arrived and provide information that the company has facts on. Do not delude the people in thinking, the situation is worse or better than what the facts actually say, but be honest and clear about the situation. It is a good idea to have a mediator around if the company decides to have a verbally conversation that is one on one with a family member, and this will prevent any rumors as well. Inform the employees and families that it is in the best interest for the company and the families of the miners to avoid answering questions to the media. When speaking to the media without knowing all the facts or going based on what you heard without fully researching the situation can lead to panic and discomfort for the families and loved ones. For instance, in the situation the media publicly announced on August 26, 2010 that it would take the rescuer’s four months to rescue the miners. However, According to msnbc.com news services (2010), Health Minister Jaime Manalich said, they would not be rescued before the Fiestas Patrai’s,† and Fiestas Patrai’s is Chiles Independence Day celebration, held on September 18. That is only forty-four days, and a big difference from 120 d ays. Preventing the media from misleading reports can be achieved by avoiding the media. The company should offer counseling to the families and co-workers to help deal with this situation, and provide any assistance to make this incident go smoother for them. After the company has all the facts and knows what they are facing then have a reputable spokesperson address the media, family, friends, and co-workers with all the facts at hand. This will help all parties understand what they are in for and to start dealing with the situation at hand. Keeping the all parties informed throughout the rescue process though meetings or the media is important. Having somebody from the company to go to the miners loved ones face to face or calling them to ensure that they are well, is a great way to provide answer to the individual parties. This situation affects everyone, families, friends, co-workers, and global. It is never easy to try to control and sustain a crisis like this but avoiding rumors, which are usually just opinions from others around, can help everybody. The miners loved ones just have one care in the world, that is getting them out safe and healthy, and that is all that should matter, but sometimes the media likes to turn embellish the truth. The important thing in this type of situation is too continual to provide information to all parties so they are properly informed. It is better to hear from the people who are actually in the rescue process rather than an outside source. This will provide all parties some ease to their pain even if it is not good information; at least they understand the situation. Reference msnbc.com news services. (2010). Drill breaks through to trapped Chilean miners. Retrieved from http://www.msnbc.msn.com/id/39558833/ns/world_news-americas/t/drill-breaks-through-trapped-chilean-miners/

Explaining exceptions to Dollo’s Law

Explaining exceptions to Dollo’s Law Explaining exceptions to Dollo’s Law a review of the concepts of constraint and contingency. In 1890 Louis Dollo a Belgian palaeontologist, came up with the theory that evolution is irreversible, expanding on the work of Edgar Quinet, a historian who had first pondered this theory (Chopra Rogers, 2013) . Thus explaining that the constraint of evolution that it is irreversible and if certain traits are lost this effects the contingency of evolution, thus past changes having an effect on the present and future of the species, this could by chance may or may not have an effect on the re-evolution of certain traits. The theory states that evolution is irreversible because of the structures and functions lost in the line of evolution cannot return in the lineages that they were once lost in e.g tails in our monkey like ancestors. This therefore suggests that genes formally required to code for adaptive traits during selection pressures will become non-functional when selection pressure is low or non-existent (Marshall, et al., 1994). The repercussions of this are that any trait coded by these genes will be lost forever and cannot ever occur again in the same lineage according to Dollo’s law (Marshall, et al., 1994). In recent times many papers have been published that have disputed this law. There has been some work done on seeing if the constraints of evolution hinder further adaptation and whether this can either facilitate or hinder the re-emergence of the original/ancestral trait (Yedid et al. 2008). This essay will look at some of the cases where this law potentially does not apply and discuss how relevant Dollo’s law is in biology, and if it is relevant at which point does the law either become to ambiguous or too specific. We will discuss Dollo’s law at two different bases; the Genetic and Morphological. Under Dollo’s law the genetic basis of this is that if a gene is lost due to natural selection and bred out of a population, the trait coded by the gene is lost and cannot be regained in the same lineage over evolutionary time. A Study to test the genetics of Dollo’s law was tested on the genome coding for the sex combs in Drosophila bipectinata and its close relative Drosophila malerkotliana (Seher, et al., 2012). The study found that some the genes that code for sex comb may alter the structures dramatically (even in a single inversion) and some that had multiple inversions of the chromosomal structure which had no difference in the sex comb morphology. They then suggested that Dollo’s law should follow molecular pathways rather than just the genes that code for them. This is due to many genes being regulatory genes, which can sometimes when activated; open up many pathways to code for different cellular processes. This can then have an effect in gene expressio n and therefore a trait previously lost in evolutionary time is now being expressed due to these â€Å"nexus† regulatory genes (Seher, et al., 2012). The can be demonstrated in another experiment where mouse inductive signals that gave rise to stem cells providing teeth, where cultured with graphs of chick oral dermis. The result found that the Chicks oral tissue actually started to form enamel organs and even in some case small malformed teeth (Marshall, et al., 1994). In a review published by Bull Charnov it says that In relation to irreversibility there are two generalisation from there analysis. 1) â€Å"selection of intermediate phenotypes is critical to evolutionary transitions whenever the two phenotypes are so different that multiple mutations are required to change from one to another† (Bull Charnov, 1985) , and 2) â€Å"a second principle common to several examples is that the genome may progressively accommodate a character state the loner it is maintaine d† (Bull Charnov, 1985). These two generalities the summary was that irreversible evolution is founded on the dependence of the biological details of the system, with some more general rules that apply at a much less focused level. The constraints with looking at the genetic level are that we are looking literally â€Å"under the microscope† and it is fine picking each detail of gene selection and deletion and applying this to Dollo’s law. But as said before genes can take many pathways due to nexes regulatory genes, so who is to say that a feature i.e. eyes lost in a cave fish (speaking hypothetically) came back in a recent form but using different genes to cause the eye. Is this against Dollo’s law? Or because of the different genetic pathway it is just a natural progression in evolution. Using morphology as a basis with regards to Dollo’s law it states that any morphological trait that is lost in a lineage cannot ever be re-expressed for example the hind legs in cetaceans. We cannot talk about morphological exceptions to Dollo’s rule without mentioning Atavism. Atavism by definition is a revision/reappearance to an ancestral characteristic previously lost in the evolutionary pathway (Biology-online, 2012). Atavisms arise normally due to a gene recombination or a gene mutation that enables a previous trait to be expressed (Hall, 2010). Hind leg extension in vertebrates has been well documented. In a study by Bejder Hall, they mention atavisms and the development of limb bud in cetaceans, snakes and legless lizards (Bejder Hall, 2002). They aren’t as rare as one might think this is due to all these animal species having being evolved from limbed ancestors, and as previously mentioned that genes can code for a multiple of different functions. Atav isms in whales normally occur in the rudiments of the pelvic girdle, the best case of this has been found in sperm and blue whales. The incident rate of atavisms in adult sperm whales is about 1:5000 (Bejder Hall, 2002). In the Individuals found the atavisms skeletal processes are found to be almost complete, even both hind limb have been found in a female humpback whale when normally present is cartilaginous femur (Bejder Hall, 2002). Because these vestigial limbs actually have no function can these actually be considered against Dollo’s law? Or because that previously forgotten traits are being expressed does that counter Dollo’s law? Another morphological feature that contracts Dollo’s is re-evolution of shell coiling in gastropods (Collin Cipriani, 2003). The trait was thought to have died out around 10mya but a study has shown that It can be re-evolved using the same genes that gastropods has at that time. There are two hypothesis put forward by this ide a; either that genes that signal for shell coiling have a number of function have been kept in there entirety, or that Trochita has developed a new pathway to gain the coiling trait completely different to its ancestor (Collin Cipriani, 2003). There has been evidence to support the second theory due to the coiling being superficially different to other gastropod species (Collin Cipriani, 2003). Finally an example that is a little closer to home is that there is new evidence of muscle reversions in the primate phylogeny. There have been 220 character state changes that are optimised in the parsimonious 28 of there have been evolutionary reversions, 6 of these have through evolution have contributed to human musculature and 9 of these have directly gone against Dollo’s law (Diogo Wood, 2012). The one particular case of violating of Dollo’s law for muscle reversion is in the subtribe hominina. In this case both the rhomboideus major and rhomboideus minor muscle are fou nd in an ancestral clade. This was then lost and the Rhomboidus muscles became the more distinct muscle in the Cercopithecinae, the ancestral muscle formation then has re-appeared in the Hominina there by going against Dollo’s law (Diogo Wood, 2012). this constant muscle evolution and re evolution causing the muscle to constantly re configure in primate to truly go against Dollo’s law at both eh morphological and genetic level there must be the same genetic pathways and selection pressures present to make this change a selective and adaptive advantage to truly call this change re-evolution. In summary to this review all of the studies all show great strengths and flaws with the methods and rules abided by in Dollo’s law. Constraints and contingency way heavily on if Dollo’s law is applied, because pathways may be constrained but if they actually help the re evolution of a trait there still may not be a selection pressure for these and this does not apply with the constraints of evolution, there-fore if there is no selection to me it feels like a random mutation with no beneficial attributes to the animal’s evolution. Law I feel is a strong word to use because with law there needs to be the same degree of lenience with this. This is due to papers on the genetic level saying that if the same pathways are used this means that this is against Dollo’s law, but if the same trait appears again but using a different pathway this does not, even if the new trait is a functional advantage. I believe the only way that a species can truly re-evolve traits is that the trait that has been re-evolved needs to be on a functional basis. The functional basis is that under Dollo’s law even if a limb has arisen That limb would need to be functional i.e. have a selection pressure causing this to be an advantage evolutionally. This is the only way that I can see of being able to out rightly say if something is against Dollo’s law. Word count: 1565 References : Bejder, L. Hall, B., 2002. Limbs in whales and limblessness in other vertebrates: mechanisms of evolutionary and developmental transformation and loss. Evolution Development, 4(6), pp. 445-458. Biology-Online 2014. Atavism definition from Biology-Online.org. [ONLINE] Available at: http://www.biology-online.org/dictionary/Atavism. [Accessed 27 February 2014]. Bull, J. Charnov, E., 1985. On Irreversible Evolution. Evolution, 39(5), pp. 1149-1155. Chopra, S. Rogers, K., 2013. Dollos law (biology) Encyclopedpia Britanica. [Online] Available at: http://www.britannica.com/EBchecked/topic/168293/Dollos-law [Accessed 24 febuary 2014]. Collin, R. Cipriani, R., 2003. Dollos Law and the re-evolution of shell coiling. Proceeding of the royal society of biological sciences, pp. 2551-2555. Diogo, R. Wood, B., 2012. Violation of Dollos Law: Evidence of muscle reversions in primate phylogeny and their implications for understanding iof ther intigeny evolution, and anatomical variations of modern humans. Evolution, 66(10), pp. 3267-3276. Hall, B., 2010. Atavisms. [Online] Available at: yadda.icm.edu.pl/yadda/element/bwmeta1.elementaab2/main.pdf [Accessed 27 02 2014]. Marshall, C., Raff, E. Raff, R., 1994. Dollos law and the death and resurrection of genes. Proceeding Of The Natural Academy Of Sciences Of The United States Of America, Volume 91, pp. 12283-12287. Seher, T. et al., 2012. Genetic Basis of a Violation of Dollo’s Law: Re-Evolution of Rotating Sex Combs. Genetics, 192(2), pp. 1465-1475. Yedid, G., C. A. Ofria, and R. E. Lenski., 2008. â€Å"Historical and Contingent Factors Affect Re-Evolution of a Complex Feature Lost during Mass Extinction in Communities of Digital Organisms.† Journal of Evolutionary Biology 21, no 5. pp 1335-1357.

Advantages Of Downloading Copyrighted Philosophy Essay

Advantages Of Downloading Copyrighted Philosophy Essay Copyright is a form of protection, by which we can protect the things that weve typed or manufacture and development of rules specifying how published. In these days we can download many things through the Internet without the knowledge of its reference and without paying for it. In this report I will highlight aspects of ethics about downloading copyright and its result, I will give a final suggestion to the dilemma. BACKGROUND Nowadays, we can see a lot of people who download many things without taking into account the copyright and that thing is against the low because they infringe on the rights of others. According to what Louis Kroeck says It is not illegal to download copyrighted material as long as you have the consent of the authority. (Kroeck, 2012) Some sites are adding some of the articles or songs and claim that with the consent of the author, but in fact the opposite, which leads to legal prosecution for fraud they had and infringement on the rights of others. P2P network is the largest example of websites or software that allows the user to download any music for free from the Internet and exchanged over a single network. And the most famous of these sites are Limewire, forstwire, and Napster. Napster allows users to download the corresponding version of pedigreed free. People turn to music download sections, some books and other online without regard to copyright and to shorten the time and save money or indifference to it. According to what Max Baldwin says In fact, 95 percent of music downloads from the internet are illegal downloads and only less than five people in the world have been arrested for illegal downloading as opposed to the millions of people that download illegally. (Baldwin, 2010). Resulted in the roping the idea of some sites that are developing some of the articles and to the loss of musical copyright, respect the articles of the law that we must follow. ETHICAL DILLEMA Advantages of downloading copyrighted:- There are things around us may be unethical, but gave us some of the pros, the problem of downloading copyright where a small number of pros are: Shortening the time, where we can find any song or article in any site and downloaded quickly on your computer and it saves us going to the songs stores and libraries. So we dont have to wait for delivery. Save money, where we can get on any album or a book or other for free, which is in fact a precious. So we will not paying for it. Readily available, Sometimes we want to buy a song at the night and the store is closed, so we can get it from the internet. And sometimes some albums or books that are not available in the market and can be found in the Internet.it will be available in website all the time. Disadvantages of downloading copyrighted:- There are many negatives for downloading copyright problem and these are some of them: Illegal and immoral, Known you when you download music or article without the author allow it, you are breaking the law, which allow to your exposure to legal repercussions. Effects on Music Industry, According to ehow.com According to ThinkQuest.org, every year the music industry loses approximately $4.2 billion to piracy worldwide. Musicians, too, rely on the sale of records to make a living, which in turn enables them to continue to be able to make music. (What Are the Disadvantages of Downloading Free Music From the Web to Play on Your Own PC or CDs? Read more: What Are the Disadvantages of Downloading Free Music From the Web to Play on Your Own PC or CDs? , 2012) Virus and Spyware and hacked program, when you download anything the subject your downloading can carry viruses and thats can affect your computer, some viruses can damage your computer completely and you can never use the computer again. Therefore, the question in the dilemma will be is downloading from the internet immoral? THEORETICAL RESPONSE Consequentialism:- Consequentialism refers to the type of philosophy implied in the principle of ethics and its aspects. In essence consequentialism means that the morally right action is the one with the best overall consequences (Grimmelmann, 2009). This means that the end results justify the means through which it is conceived. In case of downloading copyrighted infringement, the first thing is to establish as the downloaded material is right or wrong and does it possess a greater risk if it is downloaded illegally (or infringement). This theory ponders on the fact that is the result obtained greater in theory than from the result of violation. The second important aspect of this theory is that whether that the result obtained due to the downloaded material cannot be obtained while violating lesser or something that is even less than that (Warwick, 1999). This theory also ponders on the fact that copyright law is basically enacted to obtain information and promote creative activities. Although it is good to do so, these are not moral obligations that must be followed till the end. In light of this theory, downloading copyrighted material is not immoral. Non-consequentialism:- Non-consequentialism takes on a different approach in this regard and this philosophy directly implies on the moral obligations of the action itself. According to this philosophy, some kinds of actions or activities are wrong in themselves (Woodcock, 2000) and not just wrong because they have negative results or consequences. Speaking in terms of the issue of downloading copyrighted material, this theory discourages this kind of action. It is because copyrighted materials are the private property of some individuals and the decision lies solely with them that whether they want to distribute that material or not. This theory even characterizes the act of downloading copyrighted material as illegal or theft. There are certain boundaries of freedom that one can enjoy as long as he or she is not trampling on others. The issue of downloading copyrighted material directly relates to this theory as people use their downloading freedom a little too much (Grimmelmann, 2009) and end up downloading copyrighted material for which they would have to pay something in reality. This is the reason that this theory categorizes this act of downloading copyrighted material as immoral. Virtue-based ethics:- This theory is more based on the relativity of action in terms of a virtuous person than the end result of the activity itself. In basic terms, this theory means that a right action or ethical action is one which the virtuous person would do under the same circumstances. Regarding the case of downloading copyrighted material, this theory takes an interesting twist. Talking about the issue of downloading copyrighted material, this theory does not hold any final implication (Baldwin, 2010) as to whether this action can be considered right or wrong. If a knowledge source is being downloaded that can certainly benefit a lot of people, then this theory might shift in this case favor because a virtuous person is one who looks for the greater good. However, if the downloading of copyrighted material is hurting the original owner and fewer benefits in this approach, then this theory might be against this feature. So it really depends upon the type of circumstances that is present at that time. PERSONAL EXPERIENCE In my opinion, the ethical issue related to downloading copyrighted material lies solely with the subject of material being downloaded. There are tons of different things that are present on the internet and are copyrighted material (Woodcock, 2000). These might include movies, music, games, books, magazines and computer software. I have to agree more with more of virtue based ethic here because this theory truly reflects on my thinking of this matter. The type of material that is being downloaded defines as to whether my action is moral or immoral and does downloading it provides me with any greater good (Grimmelmann, 2009). For example, a book that is quite expensive in the market and is out my budget range is available free on the internet; I will download it for my use. This is because I know that this book can help me and other fellow students in achieving personal growth which otherwise would have not been possible if I were to buy it. Virtue-based ethics theory discusses the actions of a pious or virtuous person in the same circumstance and according to me; I fall in this category and have a same outlook. CONCLUSION There is a never ending debate present on the issue of downloading copy righted material where one side strong-fully objects to it while the other side posts its benefits. There are certain advantages and disadvantages of this activity (Woodcock, 2000) and none of them seems to outweigh the other. In the end, it all depends upon the fact that whether the action performed has resulted in any major losses or trampling of moral issues. The major thing to lookout for here is that whether the results obtained from downloading copyrighted material possess greater good. If copyright infringement poses a serious threat to the hard work of an individual, then without a doubt it is considered wrong as the person rightfully deserves the credit for his work by paying him or her. On the other hand, if it has more benefits than risks then it can be considered an acceptable act.